U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX1050129: GSM1704352: MK_059; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 9.7M spots, 492.3M bases, 172.6Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Refining brucellosis diagnosis by blood transcriptional profiling.
show Abstracthide Abstract
Diagnosis of brucellosis remains challenging for several reasons, including lack of culture sensitivity, nonspecific symptomatology, and high prevalence of positive serology in endemic areas. The main objectives of this study were to identify blood biomarkers specific to brucellosis compared to other endemic infections and to monitor changes in blood biomarkers during treatment. To obtain a global profile of the disease, we employed RNA sequencing (RNAseq) of whole blood RNA to measure host response against brucellosis infection in patients from Macedonia and Spain. Long-term follow up of patients was used to classify patients as having acute or chronic/reinfection brucellosis and as treatment responders and non-responders. We observed distinct gene expression differences between samples from acute brucellosis and control donors. The magnitude of gene expression changes was associated with antibody titers determined by standard serological tests for brucellosis, including the rose Bengal and standard agglutination tests. The expression signature characteristic of acute brucellosis was also different from that of subjects with leishmaniasis. In depth integration of clinical data and serological findings with our gene expression data will be performed to provide insight into cellular and molecular mechanisms of brucellosis infection. Overall design: Whole blood from 169 subjects including 105 patients with suspected brucellosis, 17 patients with leishmaniasis, and 47 healthy controls.
Sample: MK_059
SAMN03762281 • SRS954415 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was isolated using MagMax for Stabilized Blood Tubes RNA Isolation Kit (Ambion, TX) and RNA sample was globin reduced wit GLOBINclear (Ambion, TX) according to manufacturer’s instructions. Libraries were prepared from globin reduced RNA samples using the Illumina TrueSeq RNA Sample Preparation kit according to manufacturer's instructions.
Experiment attributes:
GEO Accession: GSM1704352
Links:
External link:
Runs: 1 run, 9.7M spots, 492.3M bases, 172.6Mb
Run# of Spots# of BasesSizePublished
SRR20527039,739,335492.3M172.6Mb2015-06-16

ID:
1525261

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...